Solid Phase Synthesis of Thymopoietin-(32-36) Using Adpoc Amino Acids and its Biological Activity+

An exemplary peptide has been synthesized for the first time by an automated solid-phase instrumentation which utilized the new Adpoc (1 (1-adamantyl) -1 -methylethoxycarbonyl-) group for protection of the a-amino groups of the amino acids. The pentapeptide, thymopoietin-(32-36) was synthesized, and shown to be pure by criteria including HPLC. Using a Cytoxan (cyclophosphamide) model system for suppression of a primary, hemolytic, humoral, antibody level, thymopoietin-(32-36) showed low activity, and reversed the suppressing effect of Cytoxan. This biological activity apparently is the result of cummulative effect.